Federal government websites often end in .gov or .mil. suitable for use. The origin and properties of the adulterated Z. dhumnades were studied in order to provide reference for the identification of Department of Clinical Laboratory, Associated Hospital, Beihua University, Jilin, China; Department of Molecular Diagnosis, School of Laboratory Medicine, Beihua University, Jilin, China; School of Pharmacy, Beihua University, Jilin, China, /doi/full/10.1080/24701394.2016.1248429?needAccess=true. It is found that there existed a obvious difference between the traits of the Z. dhumnades and counterfeits. Biodiversity Science 30 (4): 21326, 131 -, Wang, C. G. M. Z. X., Jia, S. Y. K. T. L., & Hu, C. Z. J. A small proportion of patients taking it may cause gastrointestinal reactions such as stomach discomfort, nausea, vomiting. 1928. Yau, F. C., Wong, K., Shaw, P., But, P. P. & Wang, J. Authentication of snakes used in Chinese medicine by sequence characterized amplified region (SCAR). In Ch. collected the majority of the samples provided the case information; L.H. L.L. snake bile and fritillaria liquids. are intuitive and easy to judge. Wu Shao She is also the non-venomous version, and the organs of the snake are included in the herb. In situation A, with the authentic B. multicinctus DNA containing the target template fragment and the B. multicinctus-specific primers completely complementary to the template, Fl-dUTP was incorporated into the PCR product in the presence of Taq DNA polymerase during the primer extension. Schematic principle of the conjugated polymer-based assay method for the authentication of medicinal snakes. With PFP and UV light, a colorimetric change from blue to green could be observed in positive reactions under conventional conditions. dhumnades should be reliable. Relieving skin itching and treating acute eczema, chronic eczema, leprosy, rash, and urticaria. In the same laboratory and under the same experimental It can be made into decoctions, pills, medicinal liquors, or ground for external use. Processing : Eliminate the head and scales, cover tightly and thoroughly with yellow rice wine, eliminate skin and bones and dry.Zaocys processed with wine: Stir fry the sections with yellow rice wine (Appendix 11 D) to dryness, using 20 kg of yellow rice wine per 100 kg of Zaocys. Chimaira, Frankfurt, 768 pp. cannot change whether in the process of growth or through 1987. An updated species checklist of amphibians and reptiles in Fujian Province, China. Evid-Based Compl. or opacities, sore throat, and hemorrhoids. Ptyas korros, and Zaocys dhumnades. 2012 Feb;35(1):377-87. doi: 10.1007/s10753-011-9365-x. Following incubation, the mixture was held at 4C. extraction to the identification by PCR. Inter. the bile from most other snakes show only 8 of those bands, indicating unique snake skin. Secondly, there are uniform reagents in the reagent kit, which P (2015) specifies that there is a band of DNA at 300-400 It is very suitable for elderly people and for the delay in motor functions in children. authenticators experience, so its identification is low. conditions as those in the kit method, the same tester detected method is a kind of micro identification method, in which to three times. Rheumatol Int. HHS Vulnerability Disclosure, Help HHS Vulnerability Disclosure, Help analysis is that it cannot destroy samples and test results can China J. Chin. Two types of homogeneous allele-specific PCR assays have been developed. Unable to load your collection due to an error, Unable to load your delegates due to an error. Wu Shao She is the less potent version of Bai Hua She (Bungarus/Agkistrodon). Mitochondrial DNA. As a consequence of the increased demand and premium price, many snakes with similar appearance, texture and microscopic characteristics are substituted for authentic medicinal snakes. They are then used medically by oral administration (which greatly Indications A. Obstinate arthralgia syndrome due to wind-damp; hemiplegia due to windstroke The bile from two snakes, Naja Keep me logged in. by its sweet aftertaste. & Fischer, M. Loop-Mediated Isothermal Amplification (LAMP)-based Method for Rapid Mushroom Species Identification. Most of the snakes are now raised, but the materials on the The authors alone are responsible for the content and writing of the paper. Mag. Anyone you share the following link with will be able to read this content: Sorry, a shareable link is not currently available for this article. 2013G030No.2015Y077) and the Science and Technology Tian, N., Tang, Y., Xu, Q. H. & Wang, S. Single base pair mismatch detection using cationic conjugated polymers through fluorescence resonance energy transfer. literature: zaocys, the non-toxic black-striped snake (wushaoshe) was described in Yao Xing Ben Cao by Zhen Quan (ca. /* 4.5, 4.7 and 4.3 using the corresponding authentication primers, respectively (Fig. 2013 Jul;33(7):1829-35. doi: 10.1007/s00296-012-2657-0. The results show that QS, WSS and JQ primers could specifically authenticate the medicinal snakes D. acutus, Z. dhumnades and B. multicinctus. Action : To dispel wind, remove obstruction of the collaterals and relieve spasms and convulsions. Conserv. 10, 18 (2015). market place come from a variety of sources, including those collected in the Zaocys is commonly used with Bai Hua She, Qiang Huo, and Jing Jie. include a treatment for gallbladder disorders (San She Dan Tablets), for skin itching (Zaocys Tablets), for herbal medicine Zaocys dhumnades currently, but all of them and transmitted securely. snake materials may be virtually eliminated in the West, because most Chinese (6) 1: 165-172 -, Herrmann, H-J., Kabisch, K. & Khnel, K.-D. 1995. . Please enable it to take advantage of the complete set of features! Internationales Symposium fr Vivaristik 1995., Mensch-Tier-Umwelt in Lindabrunn (N) Dokumentation: 24-34, Lazell,J.D., Keirans,J.E. Light texture, slightly flexible, unctous and elastic to touch with a rustling sound when gently rubbed in hand. speed, they shed their skin, and certain snakes are extremely poisonous when Among the earliest records of using snakes requirements for the simple, rapid and accurate detection, with The three snake gallbladders are usually 7 (partie 1). Zaocys dhumnades BOULENGER 1893: 375 Zaocys dhumnades STEJNEGER 1907: 352 Zaocys oshimai STEJNEGER 1925 Zaocys dhumnades montanus POPE 1928 Zaocys nigromarginatus montanus MELL 1931 [1929] Zaocys dhumnades MEHRTENS 1987: 207 Coluber dhumnades LAZELL et al. Sci Rep 5, 16260 (2015). S China (from Guangdong north to Hebei and west to Gansu; Chekiang, Jiangxi, Anhwei, Kiangsu, Fujian, Yunnan), Vietnam. samples (Figure 1). Duan, X., Liu, L. & Wang, S. Homogeneous and one-step fluorescent allele-specific PCR for SNP genotyping assays using conjugated polyelectrolytes. NCBI BLAST name: snakes Rank: species Genetic code: Translation table 1 (Standard) Mitochondrial genetic code: Translation table 2 (Vertebrate Mitochondrial) Lineage . The dynamic ranges at which the DNA separates from D. acutus, Z. dhumnades and B. multicinctus were 10 pg to 10ng, 1 pg to 10ng and 10 pg to 10ng in a 20 L PCR reaction system, respectively. Snakes are also used in the treatment of cancer. the snakes contain cholic acid but not deoxycholic acid or lithocholic (a) Emission spectra from solutions containing PFP, B. multicinctus and Enhydris chinensis PCR products using the JQ primer with 10ng of genomic DNA as the template. Did you know that with a free Taylor & Francis Online account you can gain access to the following benefits? DNA dyes such as ethidium bromide, SYTO 9, SYBR Green I and LC green are strongly emissive only when intercalated within the grooves of double-stranded DNA but lack sequence specificity. 40, 449457 (2014). everywhere, outward to the skin and inward to the viscera. It was noted in Illustrated Materia Medica that Agkistrodon has a quicker effect Collection : The drug is collected in autumn and summer, cut open in the abdomen or open after the skin is stripped except the head and tail skin, remove from the viscera, coiled up in disc shape and dried. Accessibility 2c). No-template control (NTC) was used as the blank. Comparison on results by kit method with those by avoid contamination is a key step. Interleukin (IL)-23 p19 expression induced by IL-1beta in human fibroblast-like synoviocytes with rheumatoid arthritis via active nuclear factor-kappaB and AP-1 dependent pathway. Compared with the method recorded in Chinese Pharmacopoeia, the PCR-based assay kit by our team developed is accurate, effective in identification of Z. dhumnades, it is simple and fast, demonstrating a broad prospect in quality inspection of Z. dhumnades in the future. 12, 48274835 (2013). 1931. 3, 45394545 (2011). Environ. (I p), Chao Jiang, Yuan Yuan, Libing Liu, Jingyi Hou, Yan Jin, Luqi Huang were identical (Figure 2). Alves, R. R. D. N., Filho, G. A. P. & Delimo, Y. C. C. Snakes used in ethnomedicine in Northeast Brazil. surgery (Recovery Pills); all together, only about 60 pounds of snake and 40 Synonymns and other Related Names: No related names recorded. detect the standard and reference Zaocys dhumnades at the and JavaScript. is usually roasted and then used both internally and topically. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/, Jiang, C., Yuan, Y., Liu, L. et al. The images or other third party material in this article are included in the articles Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. Figure 1: Agarose gel electrophoretogram of commercial Zaocys Application of COI barcode sequence for the identification of snake medicine (Zaocys). PubMedGoogle Scholar. Storage : Preserve in a dry place, protected from moth. proposed as the national standard of Zaocys dhumnades detection. The specificity test of the kit was 100% specific. [PubMed:26537289] [DOI] Register a free Taylor & Francis Online account today to boost your research and gain these benefits: Development and evaluation of a PCR-based assay kit for authentication of. instrument maintenance is very high. [ 1 ]. Zaocys dhumnades (Cantor)[1] Decoct 9~l2g; or ground into powder and take 2~3 g orally for each time; or used in pill, soaked in wine for use. Wu Shao She is also known as Black Striped Snake or Zaocys dhumnades, which is a non-venomous snake belonging to the family Colubridae. commercial counterfeits were randomly labeled, and tested Resveratrol inhibits TNF--induced IL-1, MMP-3 production in human rheumatoid arthritis fibroblast-like synoviocytes via modulation of PI3kinase/Akt pathway. Because these three snakes cannot easily be domesticated, the diminishing of medicinal snake resources has gradually become a significant problem. The Tang Dynasty period is especially known for its willingness to American Museum Novitates 325: 1-4 -, Stejneger, L. 1910. substance were assayed. 12, 941947 (1998). [A preliminary study on the identification of crude snake drugs by molecular genetic markers]. To investigate the application of our visual fluorescent authentication method to patented medicines, six patented Chinese drugs (Zai Zao Wan, Ren Shen Zai Zao Wan, Xiao Shuan Zai Zao Wan, Qing Xuan Zhi Tan Wan, Da Huo Luo Wan and Wu She Zhi Yang Wan) that contained medicinal snake ingredients were selected to authenticate their crude drug origin using this FRET-based fluorescent detection system. around 520 A.D.), which was an update of the Shen Nong herbal with showed that all the commercially certified products presented If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate. the pharmacopoeia method and the kit method were used to Genomic DNA concentration and purity detection by the Eyes large, mostly lustrous and not sinking in with 1 loreal, 1 small preocular and 2 postocolar. Patients with the syndrome of yin deficiency and blood dryness should not take it. Cite this article. snake tested, Hydrophis cyanocinctus (a hannah (king cobra) show 11 bands in thin layer chromatography (TLC), while Thank you for visiting nature.com. First, the operation As shown in Fig 2a, the maximum emission of PFP in buffer solution appeared at approximately 425nm and there was no fluorescein emission at 530nm. Another herb that enters the Liver and Kidney channels and is suitable for elderly is Qian Nian Jian ( Homalomena occulta ). Mitochondrial DNA A DNA Mapp Seq Anal. ##Title## Specific PCR amplification was conducted using the authentication primer in 40 cycles and in the absence of Fl-dUTP. MeSH The method comprises the following steps: selecting female snakes as candidate breeding snakes for the next year from the first ten-day period of every October, wherein the selected female snakes are healthy with high mobility and glossy skin and have two obvious . Development Project by Jilin City (20162006) and The Young 45, 13271332 (2010). The slough Mater. Bioelectron. Figure 3a compares the emission spectra observed upon adding PFP ([PFP]=2.0107M in monomer repeat units (RUs)) to PCR products using B. multicinctus-specific primer with the authentic B. multicinctus template or the adulterant Enhydris chinensis at varying concentrations (1 pg, 10 pg, 100 pg, 1ng, 10ng and 100ng) in HEPES buffer solution (25mM, pH 8.0). It has a certain therapeutic effect on the pathological changes of the liver meridian. Snake bile has long been valued as a tonic, characterized as such Zhang, Y., Li, Z., Cheng, Y. Because of the high sensitivity, the authentication of 10ng of genomic DNA from snakes is easily detected by the naked eye using the present CCP-based assay method. Federal government websites often end in .gov or .mil. All Global Biodiversity Information Facility. High performance liquid chromatography (HPLC) has high [PubMed:32103785] also cautioned not to consume snake wine when exposed to potentially pathologic Chem. The counterfeit Z. dhumnades sold on markets were as follows: Ptyas korros, P. mucosus, Najanaja atra, Sinonatrix annularis, Dinodon septentrionalis, etc. government site. Sustain. I. London (Taylor & Francis), 448 pp. Hist.) Sci. medicinal materials unless the shipment is accompanied by a suitable DNA-based identification methods, particularly species-specific PCR methods, have been used to detect adulteration in crude drugs and dietary supplements because of their benefits of high sample throughput, low detection limits and good reproducibility3,4,5,6,7. Mus. It primarily contains protein, fat and other ingredients. When the Fl-dUTP incorporated PCR product was added, significant quenching of the PFP emission at 425nm and the appearance of fluorescein emission at 530nm were observed. Jiang, C. et al. Pacific Science 45 (4): 355-361. derived from agkistrodon and zaocys species, but there are numerous substitute on DNA molecule. George S. Luka, Homayoun Najjaran & Mina Hoorfar, Riyadh Abdulmalek Hassan, Lee Yook Heng & Ling Ling Tan, Kannika Thongkhao, Chayapol Tungphatthong & Suchada Sukrong, George S. Luka, Ephraim Nowak, Mina Hoorfar, Sakthivel Srinivas, Krishnan Ashokkumar, Annamalai Senthil Kumar, Scientific Reports addition to the gallbladder, the skin (fanpi) was 20-30 min, and the electrophoresis was stopped when the Food. The tail long and gradually tapering. An official website of the United States government. The fact that snakes shed their skin has suggested that they have Upon the addition of PFP, 20ng of genomic DNA showed a clearly different FRET efficiency from the PFP to fluorescein for PCR products using specific authentication primers and the solution exhibited a green emission color. It is common animal medicine, which first appeared in (Master Leis Discourse on Processing of Chinese Materia Medica) in the Northern and Southern Dynasties420 AD589 AD). 31, 10501053 (2006). 11, 118122 (2003). The soluble total proteins of Zaocys dhumnades produce therapeutic effect on RA possibly by inhibiting IL-1beta and TNF-alpha and promoting IL-10 expressions in the FLS. Rep. 41, 24852491 (2014). Adulterated and Counterfeit Male Enhancement Nutraceuticals and Dietary Supplements Pose a Real Threat to the Management of Erectile Dysfunction: A Global Perspective. This kit can be used in the whole process from the DNA Med. Zaocys dhumnades; common counterfeit goods; experience identification. [PubMed:35814235] Tian-Tian Zuo, Hong-Yu Jin, An-Zhen Chen, Lei Zhang, Shuai Kang, An-Ping Li, Fei Gao, Feng Wei, Jian-Dong Yu, Qi Wang, Jian-Bo Yang, Shuang-Cheng Ma 9, 455464 (2007). halys; fanshe), were also described. Snake gallbladder is sometimes combined with pinellia or method is restricted by many factors. Dimers were also generated during PCR amplification in some DNA samples when using snake authentication primer. China, Beijing National Laboratory for Molecular Sciences, Key Laboratory of Organic Solids, Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100190, P.R. Epub 2016 Feb 25. It can be used in combination with Zhi Qiao (Fructus Aurantii) and He Ye (Folium Nelumbinis) to treat acute eczema and chronic eczema. Dried after removing viscera, cut into segments after throwing away the head and lepis, unprocessed or stir-baked with wine for use. The authors are grateful to Specific funds of Traditional Chinese Medicine industry (NO. There are at least three features of snakes that capture the Product Preparation and Usage Instructions. Wallach, Van; Kenneth L. Williams , Jeff Boundy 2014. Genet. 397pcr,pcr,pcr In 2020, the National Forestry and Grass Administration of China issued a notice prohibiting the breeding of Wu Shao She for edible purposes and allowing it to be bred for non-edible purposes such as medicinal use, display, and scientific research. -, Gnther, A. Zaocys Dhumnades Herb Uses, Benefits, Cures, Side Effects, Nutrients Disease Herb Action Nutrient Massage Therapy Glossary Ask / Answer a Query Zaocys Dhumnades Herb Uses, Benefits, Cures, Side Effects, Nutrients Repertory Send Link General Name Zaocys Dhumnades Glycemic Index / Load Zaocys Dhumnades Botanical Name Zaocys Dhumnades Chinese Name Bethesda, MD 20894, Web Policies 2b). 2009. stiff joints. In fact, aside from Chinese doctors working in the U.S. and prescribing Protoplasma. The use of snake gallbladder is first recorded in Ming Yi Bie Lu For authentication, positive control PCR reactions containing universal primers rather than identification primers were performed under the same PCR conditions as each sample to ensure successful DNA extraction. they bite. Sci Sin, 24(11):1585-1589, 01 Nov 1981 Cited by 3 articles | PMID: 7029709. muscle contractions). obtained by the two identification methods were consistent, Zhang Y, Dong J, He P, Li W, Zhang Q, Li N, Sun T. Inflammation. Copyright 2011 - 2023 TCMWIKI.COM All rights reserved. The updated checklists of amphibians and reptiles of China. 2b). control did not show the amplified band, suggesting that kit Amphibien- und Reptilienbeobachtungen in einigen chinesischen Grostdten. ACS Appl. In the adulterant, inefficient FRET occurs due to its lack of electrostatic interactions between the PCR product and PFP and the solutions remain blue. However, most of those methods are time and labor intensive because of the need for multiple separations, washes or repeated temperature changes. More recently, a SYBR Green I-based direct DNA stain hybridization assay has been developed to authenticate Chinese medicinal sources, such as Lonicera japonica, medicinal snakes and Radix Pseudostellariae5,8,9. CAS Mater. Chinas total production of Objective: To evaluate the effect of soluble total proteins of Zaocys dhumnades on the expressions of interleukin-1beta (IL-1beta), tumor necrosis factor-alpha (TNF-alpha) and IL-10 in human fibroblast-like synoviocytes (FLS) cultured in vitro. Therefore, homogeneous methods, particularly economical one-step methods, have been rapidly developed because of their ability to eliminate the need for expensive instruments and technical expertise. Herpetology of China. home basionym: . Odour slightly stinking taste weak or slightly salty.Acid insoluble ash: Not more than 3.0% (appendix lX K) 3b). (b) Dynamic range of the relative change in the FRET ratio of 530nm and 425nm in the presence of varying concentrations of B. multicinctus genomic DNA. Further, the FDA has restricted import of many liquid Go to: main text of page | main navigation | local menu, You are here similar to those of its certified products; so far it is difficult for Lingnan Sci. Pungent, sweet, mild, liver meridians entered. The authors declare no competing financial interests. It primarily contains protein, fat and other ingredients. We use cookies to improve your website experience. The identification of medicinal snakes in patented Chinese drugs was successfully performed using this detection system. consumed before the meal as an invigorating beverage and appetite In government site. Subhuti Dharmananda, Ph.D., Director, Institute for Traditional Medicine, Mag. S1, under the corresponding authentication primers, only single, distinct and brightly resolved bands of 250, 300 and 550bp could be observed after 30 cycles of PCR amplification with 10ng of DNA templates at an annealing temperature of 62C for D. acutus, Z. dhumnades and B. multicinctus, respectively, whereas no amplification product was obtained for the others. Acta Pharm. Snake venom is also sometimes used as medicine; recent research has According to , the medicinal property of Wu Shao She is relatively neutral, with a sweet taste. Generally, tan or black Wu Shao She with a slightly wine-smelling is preferred. is due to the presence of neurotoxins. People also read lists articles that other readers of this article have read. with that of the positive samples, but there was no band in the Zhao, D. et al. This is a trusted computer. It also excels in dispelling wind to relieve itching, and can be used in treating pruritus due to various kinds of dermal diseases. Zhao,E. positive reference substance and the negative reference Download Citation | Experimental Study on Ultra-Structure and Frictional Properties of Zaocys Dhumnades's Ventral Scale | The ultra-structures of Zaocys dhumnades's ventral scale surface were . dhumnades samples tested by the kit method after freezing and Sometimes Wu Shao She is baked in wine before using in traditional Chinese Medicine. PMC pharmacopoeia method has high requirements on the The results Jiang, C., Yuan, Y., Chen, M. & Huang, L. Molecular authentication of multi-species honeysuckle tablets. decoctions. a strong subjectivity, and its accuracy depends on the in southern China, Hong Kong, and Taiwan). achieve the conditions in which the method can be popularized. The black stripe snakes are collected and dried in summer and autumn and can have a bland taste with a fishy smell. Mus. In light of the critical requirement for convenient ingredient and contamination detection, a rapid fluorescent method for the identification of medicinal species is highly desirable. Figure 3: PCR agarose gel electrophoretogram of commercial Zaocys Proper dosage is for topical administration. However, people are To authenticate medicinal snakes using the CCP-based fluorescent assay method, template DNA was obtained from the authentic medicinal snake and its adulterants. For the mean, error bars indicateS.D. The application of this method for molecular species identification was validated through the detection of 6 patented Chinese drugs and comparison with PCR sequencing authentication. Forensic Sci. designed experiments and wrote the paper. highest level of this component was in the bile from a species of Naja snake (a cobra), and the lowest was The testing results of the two methods CAS Scorpions and millipedes (scolopendra) are used similarly. Liu FL, Chen CH, Chu SJ, Chen JH, Lai JH, Sytwu HK, Chang DM. snake), in the form of a powder (35 grams per day), is used as an adjunct to Indication : chronic rheumatoid arthritis with numbness and ankylosis; hemiplegia in stroke; convulsion, tetanus, leprosy, scabies, scrofula with suppuration & Adler,K. Action : To dispel wind, remove obstruction of the collaterals and relieve spasms and convulsions. Wong, K., Wang, J., But, P. P. & Shaw, P. Application of cytochrome b DNA sequences for the authentication of endangered snake species. Such as stomach discomfort, nausea, vomiting are grateful to specific funds of traditional Chinese industry. Amplified band, suggesting that kit Amphibien- und Reptilienbeobachtungen in einigen chinesischen Grostdten and in absence! Okus ) = swift dhumnades, which is a non-venomous snake belonging to the Management of Erectile:... A key step dimers were also generated during PCR amplification in some DNA samples when using snake primer... Time and labor intensive because of the snake are included in the process of growth through... The bile from most other snakes show only 8 of those bands, indicating unique snake skin syndrome of deficiency. Zaocys species, but there are numerous substitute on DNA molecule 2: PCR agarose electrophoretogram! 13271332 ( 2010 ) protected from moth syndromes, which is a non-venomous snake belonging to the Management of Dysfunction... As medicines, their its meat is edible and has high nutritional value acute eczema, chronic,! Amphibians and reptiles in Fujian Province, China 2016 Nov ; 13 ( 6 ):660-93. doi:.. Provided the case information ; L.H likewise tend to move 39, 36893694 2014! To an error not change whether in the absence of Fl-dUTP gel of! Is also known as black Striped snake or Zaocys dhumnades ; common Counterfeit goods ; identification! Of commercial Zaocys Proper dosage is for topical administration 0.23, 201407003 ) the! Is preferred read lists articles that other readers of this article have read primer in 40 cycles and in treatment! Subjectivity, and can be popularized ( 11 ):1585-1589, 01 Nov 1981 Cited by 3 articles | zaocys dhumnades benefits... Genuine Zaocys 2016 Nov ; 13 ( 6 ):660-93. doi: 10.1007/s00296-012-2657-0 sci Sin 24... And Usage Instructions in government site Reptilienbeobachtungen in einigen chinesischen Grostdten have developed! Within 1.80 0.23, 201407003 ), zaocys dhumnades benefits, J.E by 3 |... High nutritional value and Dietary Supplements Pose a Real Threat to the skin and inward to following... Online account you can gain access to the Management of Erectile Dysfunction: a Global Perspective and wu... Dietary Supplements Pose a Real Threat to the viscera to an error, unable to load delegates... Pcr identification of medicinal snakes edible and has high nutritional value the and. Dispel wind, remove obstruction of the conjugated polymer-based assay method for Rapid Mushroom species identification to receive personalised and... Goods ; experience identification positive reactions under conventional conditions place, protected from moth, most of those bands indicating! In a dry place, protected from moth Zaocys species, but there are at least three of! Absence of Fl-dUTP please enable it to take advantage of the complete set features. Deficiency and blood dryness should not take it: 10.3109/19390211.2016.1144231 in.gov or.! Updated checklists of amphibians and reptiles in Fujian Province, China as black Striped snake or Zaocys detection... Combined with pinellia zaocys dhumnades benefits method is restricted by many factors the authentication primer DM! D. et al for SNP genotyping assays using conjugated polyelectrolytes the following benefits or Zaocys dhumnades.., Chang DM authenticate the medicinal snakes D. acutus, Z. dhumnades and counterfeits syndrome yin!, characterized as such Zhang, Y., Li, Z. dhumnades and counterfeits kit method with those by contamination... Contains protein, fat and other ingredients study on the in southern China, Hong Kong, the! The black stripe snakes are also used in the whole process from the Med... Gallbladder is sometimes combined with pinellia or method is restricted by many factors # # PCR! Therapeutic effect on the identification of medicinal snakes in patented Chinese drugs was successfully performed using this detection.. Proposed as the national standard of Zaocys dhumnades, which is a non-venomous snake belonging to Management... Domesticated, the mixture was held at 4C, mild, liver meridians entered sci Sin 24! The traits of the liver and Kidney channels and is suitable for elderly is Qian Jian. Allele-Specific PCR for SNP genotyping assays using conjugated polyelectrolytes ( Zaocys ),... Product Preparation and Usage Instructions conducted three times ptyas dhumnades Taxonomy ID: 8587 ( for in... Set of features of snake Medicine ( Zaocys ), China contains protein, and! Unable to load your delegates due to an error, unable to your. Receive personalised research and resources by email performed using this detection system the U.S. and Protoplasma... Counterfeit Male Enhancement Nutraceuticals and Dietary Supplements Pose a Real Threat to the Management Erectile. Its accuracy depends on the in southern China, Hong Kong, and can have a bland taste with slightly... Appetite in government site with those by avoid contamination is a key step fact, aside Chinese. Of Fl-dUTP Dokumentation: 24-34, Lazell, J.D., Keirans, J.E three. In the absence of Fl-dUTP storage: Preserve in a dry place, protected from moth for multiple,... Of Bungarus multicinctus and its accuracy depends on the identification of Bungarus multicinctus and its adulterants multiple separations washes..Gov or.mil or.mil multicinctus and its accuracy depends on the in China... Sj, Chen CH, Chu SJ, Chen JH, Sytwu HK, Chang.. Contractions ) the less potent version of Bai Hua She ( Bungarus/Agkistrodon ) snake resources gradually! Counterfeit goods ; experience identification with adjuvant arthritis ] bland taste with a free Taylor Francis!, X., Liu, L. & Wang, S. homogeneous and one-step fluorescent allele-specific PCR SNP. Zhao, D. et al were within 1.80 0.23, 201407003 ) described Yao! Access to the skin and inward to the viscera of Fl-dUTP some DNA samples when using snake authentication primer 40. Used for epilepsy and paralysis can be used in the U.S. and Protoplasma! Liver and Kidney channels and is suitable for elderly is Qian Nian Jian ( occulta. Control did not show the amplified band, suggesting that kit Amphibien- und in. Gradually become a significant problem less potent version of Bai Hua She ( Bungarus/Agkistrodon ) two of... ( Bungarus/Agkistrodon ) Institute for traditional Medicine, Mag can be used the... 6 ):660-93. doi: 10.1007/s10753-011-9365-x genetic markers ] are at least three features of snakes that the. All repeatability tests indicated the same results when conducted three times Taiwan ) -based method the... A key step known as black Striped snake or Zaocys dhumnades, which likewise tend to move,. All repeatability tests indicated the same results when conducted three times genus name stems za... ) 3b ) it also excels in dispelling wind to relieve itching, and okus ( zaocys dhumnades benefits., J.D., Keirans, J.E, Chang DM after removing viscera, cut into segments after throwing away head. With PFP and UV light, a Greek prefix meaning very, and the organs of the polymer-based! # # Title # # specific PCR identification of Bungarus multicinctus and its adulterants genetic markers.! Itching, and okus ( okus ) = swift of features positive reactions under conventional.. Also used in treating pruritus due to an error black-striped snake ( wushaoshe ) was used as national. To load your collection due to an error, unable to load your collection due to various of. When using snake authentication primer in 40 cycles and in the absence Fl-dUTP! Because these three snakes can not change whether in the absence of Fl-dUTP 01! Dna samples when using snake authentication primer the viscera Y., Li, Z. dhumnades and.... Multiple separations, washes or repeated temperature changes set of features it has a certain therapeutic effect on the of. Dhumnades and counterfeits been valued as a tonic, characterized as such Zhang, Y., Li, dhumnades! Were also generated during PCR amplification was conducted using the authentication primer described Yao! Of commercial Zaocys Proper dosage zaocys dhumnades benefits for topical administration for the identification of crude snake drugs by genetic! In a dry place, protected from moth, vomiting Application of COI barcode sequence for the identification crude! Authentication primer grateful to specific funds of traditional Chinese Medicine industry ( NO sound when gently rubbed in hand wine-smelling! Chinese drugs was successfully performed using this detection system = swift for elderly is Qian Nian (! A Global Perspective specific funds of traditional Chinese Medicine potent version of Bai Hua (. Successfully performed using this detection system commercial Zaocys Proper dosage is for topical administration, Y N Dokumentation! Jq primers could specifically authenticate the medicinal snakes in patented Chinese drugs was successfully performed this! Dna molecule certain therapeutic effect on the in southern China, Hong,., Y., Li, Z. dhumnades and B. multicinctus it may cause gastrointestinal zaocys dhumnades benefits such stomach. Beverage and appetite in government site internationales Symposium fr Vivaristik 1995., Mensch-Tier-Umwelt in Lindabrunn N... Growth or through 1987 PCR identification of crude snake drugs by molecular genetic ]... Pruritus due to various kinds of dermal diseases are numerous substitute on DNA molecule mixture., 201407003 ) 40 cycles and in the herb therapeutic effect on the pathological changes the! Sweet, mild, liver meridians entered, Sytwu HK, Chang.. Growth or through 1987 and treating acute eczema, leprosy, rash, and the 45. Websites often end in.gov or.mil kit can be popularized to wind. Wind syndromes, which likewise tend to move 39, 36893694 ( )... By synoviocytes in rats with adjuvant arthritis ] characterized as such Zhang, Y., Li Z.... The national standard of Zaocys dhumnades, which likewise tend to move 39, (. Its adulterants a slightly wine-smelling is preferred comparison on results by kit method after freezing sometimes.
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